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Unformatted text preview: the abundance of mRNA molecules from the same tissue from two different individuals? Explain. (4 points) No. Polymorphisms between individuals could affect the hybridization efficiency to the microarray (or other steps), which would skew the ratio between two individuals, leading to erroneous ratios. . Biochemistry 309, Winter 2010 20 points 7. A labeled oligonucleotide 5-GGACTACGATTTG-3 is combined with a mixture of the unlabeled oligonucleotides (each present in excess over the labeled oligonucleotide). The mixture is boiled and then slowly cooled to room temperature. What is the complement of the unlabeled oligonucleotide? (2 points) 5-CAAATCGTAGTCC Circle the unlabeled oligonucleotide that will most likely hybridize to the labeled oligonucleotide. (2 points) ANSWER: #6 1. GGACTACGATTTG 4. G AAAT GC TAGT GG 2. AC AATCGTAGTCC 5. CCTGATGCTAAAC 3. CAAATCGTAGT GG 6. CAA T TCGT T GTCC...
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This note was uploaded on 02/16/2012 for the course BIOL_SCI 309 taught by Professor Somebody during the Winter '10 term at Northwestern.
- Winter '10