Quiz 5 key - the abundance of mRNA molecules from the same...

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Biochemistry 309, Winter 2010 20 points Quiz #5 NAME:_______________________________ TA:__________________ 1. What is a lectin? (2 points) A protein that recognizes carbohydrates 2. Are the acyl chains of fatty acids linear or branched? (2 points) Linear 3. How many acyl chains does a phospholipid contain? (2 points) Two 4. Glucose is shown below. How many chiral carbons does it contain in the linear form? How many chiral carbons does it contain in the cyclic form? (3 points) 4 in linear form, 5 in cyclic form. 5. If a transcription factor recognizes the sequence 5’-CAT-3’, order (from 1 to 3) the following major groove interactions from 5’ to 3’: (3 points) TWO POSSIBLE ANSWERS: 2 or 3 1 1 3 or 2 6. DNA microarrays can be used to compare the abundance of mRNA molecules between two samples. In class, we imagined comparing the abundance of mRNAs from healthy and diseased tissues from the same person. Could you use them to compare
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Unformatted text preview: the abundance of mRNA molecules from the same tissue from two different individuals? Explain. (4 points) No. Polymorphisms between individuals could affect the hybridization efficiency to the microarray (or other steps), which would skew the ratio between two individuals, leading to erroneous ratios. . Biochemistry 309, Winter 2010 20 points 7. A labeled oligonucleotide 5-GGACTACGATTTG-3 is combined with a mixture of the unlabeled oligonucleotides (each present in excess over the labeled oligonucleotide). The mixture is boiled and then slowly cooled to room temperature. What is the complement of the unlabeled oligonucleotide? (2 points) 5-CAAATCGTAGTCC Circle the unlabeled oligonucleotide that will most likely hybridize to the labeled oligonucleotide. (2 points) ANSWER: #6 1. GGACTACGATTTG 4. G AAAT GC TAGT GG 2. AC AATCGTAGTCC 5. CCTGATGCTAAAC 3. CAAATCGTAGT GG 6. CAA T TCGT T GTCC...
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This note was uploaded on 02/16/2012 for the course BIOL_SCI 309 taught by Professor Somebody during the Winter '10 term at Northwestern.

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Quiz 5 key - the abundance of mRNA molecules from the same...

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