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Quiz 7key - Biochemistry 309 Winter 2010 Quiz#7 NAME TA 1...

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Biochemistry 309, Winter 2010 ***MORE ON BACK*** 20 points Quiz #7 NAME:_______________________________ TA:__________________ 1. Cells use an electrochemical gradient as a source of energy for secondary active transport to concentrate glucose in the cytoplasm. You find that the concentration of glucose in the cytoplasm is 5mM when the cell is grown in medium containing 0.5mM glucose. What is the approximate Gt of the electrochemical gradient used to concentrate glucose? (Note: RT = 2.5kJ/mol) (3 points) Gt = RT ln (10) = 2.5kJ/mol x 2.3 = 5.75 kJ/mol Therefore, the energy required to concentrate glucose is -5.75 kJ/mol 2. A protein binds ligand A with a Kd of 10nM and ligand B with a Kd of 50nM. Draw the binding curves for A and B on the left axis. Draw a binding curve for a protein with cooperative binding in which the ligand acts as a positive allosteric regulator. (3 points) 3. Passive transport mechanisms facilitate equilibration of solute molecules across membranes. How does this mechanism affect the energetics of transport? (2 points)
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