Unformatted text preview: driven forward by loss of the product and the drive toward equilibrium. 5. You carry out reactions with two different enzymes. You use 5nM enzyme and varying concentrations of two different substrates. Here are the data: Enzyme A Enzyme B Enzyme A Substrate #1 Substrate #2 Substrate #1 Substrate #2 V max 50 nM-1 s-1 300 nM-1 s-1 150 nM-1 s-1 150 nM-1 s-1 K M 50 nM 300 nM 30 nM 300 nM Which enzyme is more selective for one substrate vs the other? How specific is the more specific enzyme for one substrate vs the other? (6 points) We can determine the specificity for each reaction by comparing the Kcat/Km for each substrate. Because we have the same concentration of enzyme in all of the reactions, we can use Vmax/Km directly. Enzyme A is not specific; the Vmax/Km is the same for the two reactions. Enzyme B is specific for substrate #1; its Vmax/Km is 10x higher for substrate #1 than for substrate #2....
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This note was uploaded on 02/16/2012 for the course BIOL_SCI 309 taught by Professor Somebody during the Winter '10 term at Northwestern.
- Winter '10