Quiz 12 key - The bond is strained by three negative...

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Biochemistry 309, Winter 2010 20 points Quiz #12 NAME:_______________________________ TA:__________________ 1. List four molecular forms of energy produced by glycolysis and the TCA cycle. (4 points) 1. ATP 2. GTP 3. NADH 4. FADH2 2. The G’˚ of phosphocreatine (P-Cr) transfer of phosphate onto ADP is -12.5kJ/mol. In muscle cells, if the concentration of ATP is 8mM, the concentration of ADP is 0.9mM, the concentration of creatine is 5mM and the concentration of P-Cr is 28mM, what is the actual G of phosphorylation of ATP by P-Cr in these cells? (5 points) Note: RT = 2.5 kJ/mol G = G’˚ + RT ln ([ATP][Cr]/[P-Cr][ADP]) = -12.5kJ/mol + 2.5kJ/mol x ln([8][5]/[28][0.9]) = -12.5kJ/mol + 2.5kJ/mol x ln(1.59) = -12.5kJ/mol + 2.5kJ/mol x 0.462 = -12.5kJ/mol + 1.16kJ/mol = -11.3kJ/mol 3. Describe two reasons why the gamma-beta phosphoanhydride bond in ATP is a “high energy” bond. (2 points)
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Unformatted text preview: The bond is strained by three negative charges in close proximity. The released phosphate is stabilized by resonance. 4. Describe two ways in which the hydrolysis of ATP can be used to do biochemical work. (2 points) The hydrolysis can lead to conformational changes in a protein (eg, myosin). Also, the gamma phosphate can be transferred onto a substrate and the hydrolysis of that phosphate can provide a better leaving group for the reaction. 5. Why is hydrolysis of acetyl-CoA into acetate and CoA an energetically favorable reaction? (2 points) The product, acetate is stabilized by resonance . 6. Draw the intermediate in the isomerization reaction below. (3 points) Deprotonation of C1 and protonation of the carbonyl oxygen give the C=C double alcohol intermediate. 7. Circle the atom that is reduced during the following reaction. (2 points) Biochemistry 309, Winter 2010 20 points...
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This note was uploaded on 02/16/2012 for the course BIOL_SCI 309 taught by Professor Somebody during the Winter '10 term at Northwestern.

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Quiz 12 key - The bond is strained by three negative...

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