This preview shows page 1. Sign up to view the full content.
Unformatted text preview: 3. You have a preparation of detergent-extracted mitochondria. Fractionation over an ion exchange column gives four fractions. You incubate each fraction with the following electron donors: NADH, Succinate, QH2 or reduced cytochrome C. You get the following results: Complex Normal electron acceptor (8 points) Fraction I: oxidizes Cytochrome c IV O 2 Fraction II: oxidizes Succinate II Q Fraction III: oxidizes NADH I Q Fraction IV: oxidizes QH2 III Cytochrome c To which complex (I-IV) in the electron transport chain does each fraction correspond and what is its electron acceptor in the chain? 4. What is the product of -oxidation of fatty acids? (2 points) Acetyl CoA 5. What is the building block for fatty acid biosynthesis? (2 points) Acetyl CoA (or malonyl-CoA)...
View Full Document
- Winter '10