Assignment 6 - Zhao Y and Guo W. 2009 Sec-ure nanotubes...

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Zhao Y and Guo W. 2009 “Sec-ure nanotubes with RalA and exocyst”, Nat Cell Biol. 2009 11:1396-7. 1) (1 pt) Why does it make mechanistic sense for the exocyst complex to be able to regulate the arp2/3 complex? The exocyst complex works as part of the exocytosis pathway and also recently was found to regulate actin through the Arp2/3 complex. The Arp2/3 complex is a nucleator of actin polymerization and it makes sense for the exocyst complex to be able to interact with this because actin filaments are essential for transporting exocytosis vesicles to the membrane of the cell for release. Rustom A, Saffrich R, Markovic I, Walther P, Gerdes HH. “Nanotubular highways for intercellular organelle transport” Science. 2004 Feb 13;303(5660):1007-10. 1) (1 pt) Describe one line of evidence that nanotubes are actin rather than microtubule based structures. The first experiment they used to test this was to treat the cells with trypsin-EDTA which inhibits microtubule formation and the TNTs were still able to form. However, when treated with latrunculin-B, a substance that depolymerizes F-
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Assignment 6 - Zhao Y and Guo W. 2009 Sec-ure nanotubes...

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