{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lect 2- Techniques II - Lecture 2 Cell biological...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 2: Cell biological Techniques II Make sure that you have access to Blackboard. If you do not have access to blackboard, you are probably not registered for the course. A new version of the syllabus with corrections to discussion sections and final exam day has been posted.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
FRET Fluorescence Resonance Energy Transfer (FRET): Used to show not just co-localization (200 nm optical limit), but direct interactions. If two fluorophors are within ~100 angstroms (10 nm, ~ the size of proteins and macromolecular complexes), they can transfer energy to each other. Energy transfer is dependent on [distance]1/6, so distances must be very small. Illuminate at absorbance peak of one fluorophor, and measure fluorescence at emission of the second. The emission of the first fluorophor must overlap with the absorbance of the second fluorophor to transfer energy.
Background image of page 2
FRET Illuminate at absorbance peak of one fluorophor, and measure fluorescence at emission of the second. The emission of the first fluorophor must overlap with the absorbance of the second fluorophor to transfer energy.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
FRET Illuminate at absorbance peak of one fluorophor, and measure fluorescence at emission of the second.
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}