Lect 5 - Tissues II

Lect 5 - Tissues II - Wulf et al., 2003 Major junctions in...

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Lecture 5: Integrating Cells into Tissues II
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Biochemical binding experiments
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Biochemical binding experiments
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Mass Spectrometry
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Do these proteins form complexes?  Co-IP Not always a definitive test for complex formation GST GST GST VS
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Size exclusion chromatography
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Size exclusion analysis of the Ndc80 complex Wulf et al., 2003  How do we know in which    fraction each protein elutes?  How do we determine whether these   proteins are co-eluting or are in a   complex?
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Analytical ( rate-zonal) centrifugation
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What kind of complexes are present? GST VS GST
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Yeast two hybrid
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High throughput techniques have generated genome (proteome)-wide interaction “maps”
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Unformatted text preview: Wulf et al., 2003 Major junctions in epithelia: Adherens junctions Figure 6-7 Adherens junctions are major signaling as well as cytoskeletal organization centers Ben-Zeev, A. (1997) Current Opinion in Cell Biology 9 , 99-108 Major junctions in epithelia Figure 6-5 Major junctions in epithelia: Desmosomes (a.k.a. Spot desmosomes) Figure 6-8 Desmosomally-anchored intermediate filaments distribute stresses across epithelial sheets Image of keratin filaments in an epithelial cell Major junctions in epithelia and other cells Figure 6-5 Major junctions: gap junctions Figure 6-31 Major junctions: gap junctions Figure 6-32...
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This note was uploaded on 02/16/2012 for the course BIOL_SCI 315 taught by Professor Beitel during the Spring '10 term at Northwestern.

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Lect 5 - Tissues II - Wulf et al., 2003 Major junctions in...

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