1 mar 2010 molbio

1 mar 2010 molbio - Mol bio lecture notes 1 Mar 2010 what...

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Mol bio lecture notes 1 Mar 2010 what is the difference between the location of regulatory sites within prokaryotic and eukaryotic genes? TSS, upstream or to the left, negative numbers. Eukaryotes = close to TSS or farther away (physical distance) also unlike prokaryotes the binding sites can be both upstream or downstream from TSS. What is the mechanism that regulates the interaction between RNA polymerase and the general transcription factor machinery? GTF machinery binds to -10, -5 elements. RNA polymerase interacts physically and an sit on TSS What would happen if a mutation disrupted the phosphorylation site on RNA polymerase? When bound but not translated, it is untransphosphorylated. If mutated, it will not transcribe downstream gene (if not phosphorylated) What role do are HAT and chromatin remodeling proteins and what role do they play in transcription? HAT – add acetyl groups to histones Chromatin physically binds to DNA and turns it around Both aid in transcription of particular gene What is the role of the mediator complex during transcription? It mediates between specific activators and suppressors and RNA polymerase and How is the role of the 5’ cap of a eukaryotic mRNA? It adds methylated G so that a particular message cannot be degraded How do prokaryotic and eukaryotic mRNAs differing in their termination mechanisms? Eukaryotic mRNAs cause the falling off What is the role of the polyA tail at the end of a eukaryotic mRNA? To act as the buffer.
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1 mar 2010 molbio - Mol bio lecture notes 1 Mar 2010 what...

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