10.8.09 Lab 4 - Colony PCR

10.8.09 Lab 4 - Colony PCR - 10/8/09 The Ti plasmid for...

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10/8/09 The Ti plasmid for plant transformation via Agrobacterium tumafaciens The original Ti plasmid from A. tumafcaciens encoded genes that make the plant cells produce a lot of plant ‘growth’ hormones and food for the bacteria. The modified Ti plasmid cuts out the ‘virulence’ region (puts it into a different bacterial plasmid) and replaces everything between the left and right borders with genes we want to express. Agrobacterium tumafaciens Transformation of plants means that the DNA is cut out of the bacterial plasmid and inserted into a plant chromosome at a random position. Most of the soy beans and a lot of cotton and corn products that you use every day were put through the following process. The gene that was transferred into the plant either makes it resistant to a plant herbicid (Roundup) or makes the plant express a bacterial gene that is toxic to captapillars (Bt toxin). These transgenic plants have allowed farmers to cut herbicide use dramatically and
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10.8.09 Lab 4 - Colony PCR - 10/8/09 The Ti plasmid for...

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