10.15.09 Lecture - Microscopy

10.15.09 Lecture - Microscopy - 10/15/09 Practical...

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10/15/09 Practical Fluorescence Microscopy Well, sort of. Creating contrast and resolution to gain information Contrast is determined by the number of steps that can be determined between darker and lighter portions of a scene. Contrast Detecting something in an image requires that the signal be proportionately higher than the noise. This is what we commonly refer to as ‘Contrast’ in the microscope. Many of you wear sunglasses. Aside from the often pathetic attempts to score opposite sex style points (OSSPs), why do sunglasses help you see better in many circumstances Resolution Resolution relies on contrast but has a different meaning. How many spots can you pick out in each of the 5 rows are the spots change spacing. Information content in an image. Contrast and resolution. Creating contrast and resolution to gain information Brightfield and fluorescence microscopy techniques. Lenses magnify an image by taking the divergent light rays from the specimen and converging them at a further distance. How is contrast generated at the point of focus?
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This note was uploaded on 02/17/2012 for the course BIO 1421 taught by Professor Farr during the Fall '08 term at Texas State.

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10.15.09 Lecture - Microscopy - 10/15/09 Practical...

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