CHE113 07.Microscopy

CHE113 07.Microscopy - Microscopy CHE 113 Forensic Science...

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Unformatted text preview: Microscopy CHE 113 Forensic Science James T. Spencer, Copyright 2004-2008 2 Microscopy and Stains Micro means small Skopein means to see Microscopes Preparation of specimens for light microscopy 3 Sizes 4 Sizes 5 Perceiving the World 6 Brightfield Microscope Compound Comparison Stereoscopic Polarizing Microspectrophotometer Darkfield Microscope Phase-contrast Microscope Fluorescence Microscope Electron Microscope Transmission Scanning The Instruments 7 How Our Eyes Work 8 Dutch spectacle-makers (1590), Janssens, Dutch spectacle-makers (1590), Janssens, discovered that nearby objects appeared greatly discovered that nearby objects appeared greatly enlarged with lenses. enlarged with lenses. Galileo (late 1600s), based on the Janssens Galileo (late 1600s), based on the Janssens experiments, worked out a experiments, worked out a much better instrument much better instrument with a focusing device. with a focusing device. Microscopy History Janssen Galileo 9 Other Early Other Early Microscopes Microscopes Microscopy History Leeuwenhoek Microscope Hooke Microscope Late 1600s 1670 10 Later Microscopes Later Microscopes Microscopy History Pacino, 1870 Olympus (modern) 11 Microscopy History 12 Microscopy 13 How Microscopes Work 14 The most common microscope - The most common microscope - compound light microscope (LM). Two compound light microscope (LM). Two sets of lenses: ocular and objective. sets of lenses: ocular and objective. Brightfield Mic. Brightfield Mic. The total magnification: multiply The total magnification: multiply magnification of the objective lens with magnification of the objective lens with the ocular lens. the ocular lens. e.g., ocular is 10x and the objectives is e.g., ocular is 10x and the objectives is 100, total mag. will be 1000x. 100, total mag. will be 1000x. Optical system comprised of Optical system comprised of condenser, objective lens, eyepiece condenser, objective lens, eyepiece lens and illuminator....
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CHE113 07.Microscopy - Microscopy CHE 113 Forensic Science...

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