SB0011Practical Biology ILAB REPORTEXPERIMENT NUMBER: 1TITLE:INTRODUCTION TO MICROSCOPYNAMEMATRIX NO.SIGNATURETAQIEYUDDIN ABDULLAH BINDURAHMANFS20110172AARON CHANG KOK YUUNGFS20110163NURAMIRAADIBABINTIZANURIFS20110144NURPRETTYBINTIMUHAMMADFS20110129DATE OF EXPERIMENT: 27 AUGUST 2020DATEOFREPORTSUBMISSION: 2 SEPTEMBER 2020LAB DEMONSTRATOR:MISSADEESAHAZZAHRAHBINTIJOBINTUTORIAL GROUP: TUTORIAL 6Assessment:ItemsMarks %Format____ /1Methods____ /2Results anddata____ /2Discussion____ /3Conclusion____ /1References____ /1Total____/ 10
IntroductionThe study on microscopic organisms, such as bacteria, viruses, archaea, fungi, andprotozoa need one’s to observe and recognize the organisms itself. However humaneyes are limited to see small things. Years ago, the microscope was invented byZacharias Janssen (William J Croft 2006) that help humans to overcome the problem.Now we can see the smallest things that can not be seen by naked eyes before this.A light microscope is the earliest invented microscope that is usually used in thelaboratory because it easy to handle and much more affordable than other kinds of themicroscope. It visualizes fine detail of an object by creating a magnified image throughthe focused light through the object on the glass lenses, and convex objective lensesto enlarge the image formed(Jeremy Sanderson 2019). The enlarge and clear image ofthe object can be studied and observe from the eyepiece (Jeremy Sanderson 2019).A light microscope can observe both living and non-living specimens. Though, itcannot resolve details lower than 0.2µm due to its limited resolving power that it onlycan magnify objects up to about 1500 times without losing clarity. On a specimen thatdoes not readily absorb light, colored dyes such as eosin methylene blue will be usedto create a clearer image and increase the contrast.
ObjectivesTo identify the name and function of the basic parts of the compound lightmicroscope.To learn how to focus an object (letter ‘e’) with compound microscope.To measure the diameter of the field of view of each magnification power(4x,10x,40x)Materialscompound light microscopeslidestransparentcover slipletter ‘e’ from newspaper/magazine
ProcedureAIdentification of partsA compound microscope is obtained from the the cabinet and placed securely on thetable. The following parts on the microscope are identified and labelled as figure 1.1.Eyepiece (ocular lenses): Topmost series of lenses through which an objectis viewed. What is the magnifying power of the ocular lens on your microscope.2.Body tube: Hold nose piece at one end and eyepiece at the other end;conducts light rays.3.Arm: Supports upper parts and provides carrying handle.4.Nosepiece: Revolving device that holds objectives.5.Objectives (objective lenses):a.Scanning power objective: Hold 4x lens used to view the wholeslide. Does your microscope have a scanning power objective? Ifyes, what is tits magnifying power?b.Low-power objective: Hold 10x lens used to view the object ingreater detail. Does your microscope have a low power objective?