lab report01.pdf - Assessment Items Marks Format\/1 Methods Results and data SB0011\/3 Conclusion\/1 References\/1 Total 10 LAB REPORT TITLE:1 INTRODUCTION

lab report01.pdf - Assessment Items Marks Format/1 Methods...

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SB0011 Practical Biology I LAB REPORT EXPERIMENT NUMBER : 1 TITLE : INTRODUCTION TO MICROSCOPY NAME MATRIX NO. SIGNATURE TAQIEYUDDIN ABDULLAH BIN DURAHMAN FS20110172 AARON CHANG KOK YUUNG FS20110163 NUR AMIRA ADIBA BINTI ZANURI FS20110144 NUR PRETTY BINTI MUHAMMAD FS20110129 DATE OF EXPERIMENT : 27 AUGUST 2020 DATE OF REPORT SUBMISSION : 2 SEPTEMBER 2020 LAB DEMONSTRATOR : MISS ADE ESAH AZZAHRAH BINTI JOBIN TUTORIAL GROUP : TUTORIAL 6 Assessment: Items Marks % Format ____ /1 Methods ____ /2 Results and data ____ /2 Discussion ____ /3 Conclusion ____ /1 References ____ /1 Total ____/ 10
Introduction The study on microscopic organisms, such as bacteria, viruses, archaea, fungi, and protozoa need one’s to observe and recognize the organisms itself. However human eyes are limited to see small things. Years ago, the microscope was invented by Zacharias Janssen (William J Croft 2006) that help humans to overcome the problem. Now we can see the smallest things that can not be seen by naked eyes before this. A light microscope is the earliest invented microscope that is usually used in the laboratory because it easy to handle and much more affordable than other kinds of the microscope. It visualizes fine detail of an object by creating a magnified image through the focused light through the object on the glass lenses, and convex objective lenses to enlarge the image formed(Jeremy Sanderson 2019). The enlarge and clear image of the object can be studied and observe from the eyepiece (Jeremy Sanderson 2019). A light microscope can observe both living and non-living specimens. Though, it cannot resolve details lower than 0.2µm due to its limited resolving power that it only can magnify objects up to about 1500 times without losing clarity. On a specimen that does not readily absorb light, colored dyes such as eosin methylene blue will be used to create a clearer image and increase the contrast.
Objectives To identify the name and function of the basic parts of the compound light microscope. To learn how to focus an object (letter ‘e’) with compound microscope. To measure the diameter of the field of view of each magnification power (4x,10x,40x) Materials compound light microscope slides transparent cover slip letter ‘e’ from newspaper/magazine
ProcedureAIdentification of partsA compound microscope is obtained from the the cabinet and placed securely on thetable. The following parts on the microscope are identified and labelled as figure 1.1.Eyepiece (ocular lenses): Topmost series of lenses through which an objectis viewed. What is the magnifying power of the ocular lens on your microscope.2.Body tube: Hold nose piece at one end and eyepiece at the other end;conducts light rays.3.Arm: Supports upper parts and provides carrying handle.4.Nosepiece: Revolving device that holds objectives.5.Objectives (objective lenses):a.Scanning power objective: Hold 4x lens used to view the wholeslide. Does your microscope have a scanning power objective? Ifyes, what is tits magnifying power?b.Low-power objective: Hold 10x lens used to view the object ingreater detail. Does your microscope have a low power objective?

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