BCH1 - BCH 451 Summer 2011 Exam#1 NAME Circle your problem...

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BCH 451 Summer 2011 Exam #1 NAME Circle your problem session time: M/W 1:15 T/H 1:00 Raw Score 1. Draw the predominate structures of the following molecules at the pH indicated. Be sure to include all carbon and hydrogen atoms! a. ( 1) Edman’s reagent @ pH = 5.5 or S = Sanger’s reagent at 10.1 b. (6) A = K - Q - M - P - Y - C @ pH = 11.5, OR B = C - N - W - R - M - P @ pH = 11.1 2. (0.3 each) Circle and label all functional groups or linkages in your peptide except for the peptide bond: 3. (0.2 each) Write the pKa by each functional group. It there is none, write “no pKa” by that functional group. 4. (0.5) Indicate with an arrow, exactly where B = cyanogen bromide, C = chymotrypsin, or T = trypsin would cleave your peptide. 5. (0.5) Calculate the pI of your peptide: [Show setup for full credit.] 6. Anfinsen experiment: a. (2) Describe (in complete sentences) the Anfinsen experiment. If you wish to use a diagram to supplement your description, you may do so. b.
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BCH1 - BCH 451 Summer 2011 Exam#1 NAME Circle your problem...

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