03-prok_transc2a_revised

03-prok_transc2a_revised - Transcription and its control in...

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Transcription and its control in prokaryotes 1. The DNA substrate A. Packaging and availability of the DNA B. Number and arrangement of the genes. C. Transcription control sequences. 1. Promoters 2. Sites for activators and repressors. 3. Sites for the regulation of DNA topology. 4. Sites for the termination of transcription. 2. The General Transcription apparatus. A. RNA Polymerase core enzyme. B. RNA Polymerase holoenzymes. C. NusA, GreA, GreB 3. The process of transcription initiation. A. Identifiable kinetic intermediates B. Biochemical characterization of the process. 4. Transcription elongation. A. Pausing, Sliding,Termination, and Arrest. B. Role of the RNA-DNA hybrid. 5. Transcription termination. A. Rho factor. B. Attenuation. C. Other mechanisms. 6. Examples of mechanisms of activation and repression of transcription initiation. A. The lac operon B. Regulation of nitrogen assimilation ( ntr ) genes.
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Question of RNA Pol at promoter- how does it function at synthesizing short transcripts? Changes shape Slides back and forth “Scrunches” template- RNA Pol remains the same but more DNA pushed into active pocket Structural studies of T7 RNA Pol suggest “scrunching” is answer
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Transition from initiation to elongation Point at which it begins not clearly defined In E. coli characterized by three distinct changes in RNA Pol: The σ is released- recent evidence some RNAP may retain σ as they elongate.
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03-prok_transc2a_revised - Transcription and its control in...

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