05-Lac_Repression

05-Lac_Repression - Regulation of the lac operon of...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Regulation of the lac operon of Escherichia coli The lac operon of E. coli has been studied for over 50 years, and serves as a paradigm of genetic regulation Much of our current understanding of genetic regulation comes from the study of lac regulation: Operons Promoters mRNA Negative control of transcription initiation (Repression) Positive control of transcription initiation Global regulation Permeases Allosteric regulation There is still much to be learned about the lac operon Role of the gene products in the induction process Mechanisms of catabolite repression Physiological factors controlling lac expression
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Gene Products and Regulatory Sites lacZ lacY lacA lacI LacO 1 is the strongest natural lac operator A synthetic operator deleted for the central base pair and containing a palindrome of the left half site is even stronger The lac promoter is a weak promoter requiring activation by cAMP-CAP for significant expression. In cells, the level of expression can be varied over 1000-fold by environmental signals.
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 02/17/2012 for the course CHEM 212 taught by Professor Staff during the Fall '10 term at Rutgers.

Page1 / 8

05-Lac_Repression - Regulation of the lac operon of...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online