06-Lac_Induction

06-Lac_Induction - Induction of the lac operon Lactose...

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Induction of the lac operon Lactose Permease Lactose Glucose + Galactose Allolactose Glucose + Galactose Allolactose Lac Permease ? Lac Permease ? β -Galactosidase Glucose Glucose-6-P
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Both galactoside permease and β -galactosidase play a role in lac induction. permease-responsible for uptake of inducer lacZ product β -galactosidase- responsible for formation and destruction of inducer
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To simplify the study of the induction process, gratuitous inducers may be used Gratuitous inducers are taken up by the permease, bind repressor and cause it to be released from operators, but are not substrates of β -galactosidase. Also: ONPG (for assay) XG (for indicator media)
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cAMP-CAP is the best understood of all transcription factors Homodimer of 45 kDa, undergoes subunit exchange in solution first transcriptional activator to have structure determined Activates transcription at over 100 promoters, acts by different mechanisms at different promoters Also acts as a repressor of numerous genes
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This note was uploaded on 02/17/2012 for the course CHEM 212 taught by Professor Staff during the Fall '10 term at Rutgers.

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06-Lac_Induction - Induction of the lac operon Lactose...

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