07-howcapworks

07-howcapworks - DNA bending by cAMP-CAP not important....

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Example of an oriented heterodimer experiment in vivo
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Strategy for mapping the contacts of CAP and Polymerase
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Heterobifunctional, cleavable crosslinker and “label transfer” studies Crosslinker used Results from a class I promoter, α CTD labeled Results from a Class II promoter, α NTD labeled
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Model for activation at Class I promoters Interaction of AR1 with α CTD helps α CTD bind DNA and in some cases σ 70 , increasing binding of Polymerase to promoter.
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Unformatted text preview: DNA bending by cAMP-CAP not important. When the CAP site is at -72, CAP may prevent unproductive binding by CTD in the -42 region. Model for activation at Class II promoters Interaction of AR1 with CTD increases binding of Polymerase Interaction of AR2 with NTD increases isomerization to the open complex DNA bending by cAMP-CAP plays a role...
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This note was uploaded on 02/17/2012 for the course CHEM 212 taught by Professor Staff during the Fall '10 term at Rutgers.

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07-howcapworks - DNA bending by cAMP-CAP not important....

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