08-PTS - When cells are grown in the presence of a PTS...

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When cells are grown in the presence of a PTS sugar and a non-PTS sugar, they use the PTS sugar first, then induce the enzymes necessary to use the non-PTS sugar. PTS-mediated catabolite repression and inducer exclusion are responsible for this phenmoenon. PTS also involved in chemotaxis towards PTS sugars. First example of “bacterial memory”.
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PTS sugars ( E. coli ) Non-PTS sugars ( E. coli ) Class I Class II glucose lactose TCA cycle intermediates α -methylglucoside glycerol xylose mannose maltose rhamnose mannitol melibiose fructose N-acetylglucosamine
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Glucose, mannitol, and mannose PTS of E. coli
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Trans-intramolecular phosphoryl group transfer in mannitol EIIABC “EIIA” “EIIB” “EIIC”
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EIIBC glc channel EIIBC glc (E. coli), subunit ~45 kDa, IIB domain phosphorylated on Cys421. Kinetics of glucose phosphorylation indicate a sequential mechanism, suggesting the only the complex with EIIA glc can phosphorylate the sugar. Fusion studies with phoA and lacZ suggest 8 membrane-spanning helices/monomer. Probably functions as a dimer.
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N-terminal domain of EI, showing His189 site of phosphorylation EI ( E. coli ) is a dimer of 63,489 kDa subunits. Cleaved by protease into two domains,
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This note was uploaded on 02/17/2012 for the course CHEM 212 taught by Professor Staff during the Fall '10 term at Rutgers.

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08-PTS - When cells are grown in the presence of a PTS...

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