09-CR_of_lac

09-CR_of_lac - LacL8LUV5- lac operon expression high...

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How does glucose bring about catabolite repression of the lac operon? Several key facts The expression of lac does not correlate with [cAMP]. Cells growing on glucose or lactose have about the same [cAMP]. Catabolite repression occurs in cya if also crp* crr grows on lactose. lacI needed for glucose effect. LacL8 mutation- weakens CAP site LacUV5 mutation-selected as suppressor to L8, makes -10 consensus.
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Unformatted text preview: LacL8LUV5- lac operon expression high independent of cAMP-CAP. Factors affecting diauxy cAMP-CAP required for diauxy even in lacL8UV5. Note-cAMP-CAP required for repression of lac by glucose ptsG expression is controlled by cAMP-CAP. ptsG expression correlates with CR ptsG from plasmid restores CR to L8LUV5 crp . Hypothesis for the role of cAMP-CAP in glucose-lactose diauxy...
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This note was uploaded on 02/17/2012 for the course CHEM 212 taught by Professor Staff during the Fall '10 term at Rutgers.

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09-CR_of_lac - LacL8LUV5- lac operon expression high...

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