immuno-08b

immuno-08b - Generation of antibody diversity Humans can...

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Generation of antibody diversity Humans can produce antibodies to millions of different antigens. How is this huge number of different proteins formed? Instructive hypothesis - The antibody molecule is folded around the antigen. This was excluded by denaturing Ab and then re-folding them in the absence of the Ag. Germline hypothesis - Each different antibody is formed from different genes present in the germ line. For example 10 3 H chains and 10 3 L chains could form 10 6 different antibodies. But-there aren’t enough immunoglobulin genes in the germline to accommodate this hypothesis. Somatic recombination hypothesis - Antibody diversity is generated by somatic recombination, which generates the variable regions of the immunoglobulins. Somatic mutation hypothesis - Antibody diversity arises from a high rate of immunoglobulin gene mutation during B-cell differentiation.
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Somatic recombination generating κ genes was detected by Southern hybridization studies Antibody diversity is generated by gene rearrangements
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κ light chain genes are assembled from three sets of gene segments In the mouse germ line, there are about 150 different V κ segments, each fused to a leader sequence (L) for secretion. Each L κ V κ segment is separated by about 7 kb of spacer DNA. There are 5 J
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immuno-08b - Generation of antibody diversity Humans can...

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