Lecture13_DNArepv2_2012 (1)

Lecture13_DNArepv2_2012 (1) - 1 somewhere in eastern...

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somewhere in eastern Washington… somewhere in eastern Washington…
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3 “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” J.D. Watson F.H.C. Crick Nature (1953) p 737.
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4 Biochemistry 441 Lecture 13 Ted Young February 10, 2012 Biochemistry 441 Lecture 13 Ted Young February 10, 2012 Topics for today: DNA replication: forks, eyes, and DNA polymerase. http://www.youtube.com/watch?v=49fmm2WoWBs
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5 Watson and Crick’s proposal Watson and Crick’s proposal Proof of semi- conservative replication: the Meselson-Stahl density transfer experiment.
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6 Observations of DNA replicating in vivo Observations of DNA replicating in vivo Conclusions from experiments using tritium autoradiography: 1. One double-stranded DNA molecule/chromosome for all prokaryotes and eukaryotes. 2. One replication origin per chromosome (bacteria/viruses). Multiple origins/chromosome for eukaryotes Tritium autoradiography of a circular bacterial Chromosome; Interpretation of the autoradiogram:
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Lecture13_DNArepv2_2012 (1) - 1 somewhere in eastern...

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