Lecture17_RNAP_2012

Lecture17_RNAP_2012 - 1 Remember: Examinations are...

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Remember: “Examinations are formidable even to the best prepared, for the greatest fool may ask more than the wisest man can answer.” Charles Caleb Colton-British clergyman and writer, ?1780-1832 “The highest faculty of the mind—that of tracing the analogy of unconnected observations; of evolving from the multitude of particular facts a common principle, the detection of which might recall them from confusion to system, from incomprehensibility to science” Albert Einstein Remember: “Examinations are formidable even to the best prepared, for the greatest fool may ask more than the wisest man can answer.” Charles Caleb Colton-British clergyman and writer, ?1780-1832 “The highest faculty of the mind—that of tracing the analogy of unconnected observations; of evolving from the multitude of particular facts a common principle, the detection of which might recall them from confusion to system, from incomprehensibility to science” Albert Einstein
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3 Biochemistry 441 Lecture 17 Ted Young February 22, 2012 Biochemistry 441 Lecture 17 Ted Young February 22, 2012 Today’s topic: Transcription: coupling genotype to phenotype. http://www.youtube.com/watch?v=WsofH466lqk R http://www.youtube.com/watch?v=6tqPsI-9aQA&feature=related
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4 The Holy Trinity of molecular biology: DNA makes RNA makes Protein The Holy Trinity of molecular biology: DNA makes RNA makes Protein 1. Mechanism 2. Control X RNA replication Reverse transcription (RNA viruses, telomerase)
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Transcription Transcription What is a gene? How do we identify genes in biochemical/molecular terms? Three common ways (none foolproof): 1. DNA sequence >open reading frames ( ORFs ) (but introns…; genes encoding rRNA, tRNA, siRNA, miRNA, snRNA). Genetic analysis: mutations. 2. RNA analysis >experimental: transcribed region of the gene (hybridization to an oligonucleotide array is best). 3. Binding analysis >in vitro and in vivo binding assays> regulatory regions Where does the RNA start? Where does it end? Which strand is transcribed? What non-coding DNA sequences are required to make the RNA-that, is where are the signals for transcription to start and stop? 5’ 3’ 3’ 5’ DNA non-template (coding or sense) strand top strand DNA template (non-coding or anti-sense) strand bottom strand In molecular terms a gene is defined as a region of DNA that controls a discrete hereditary characteristic . The complete gene contains protein coding
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Lecture17_RNAP_2012 - 1 Remember: Examinations are...

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