transcription_studyguide

transcription_studyguide - Study guide for Prokaryotic...

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Study guide for Prokaryotic transcription Biochemistry 452 1. Describe the subunit composition of the bacterial RNA polymerase core enzyme and holoenzyme. Which subunit(s) confer promoter specificity? 2. What is an allele-specific suppressor analysis and what are the strengths and weaknesses of using this genetic approach to identify interacting surfaces of macromolecules? 3. Draw a cartoon of the transcription elongation complex showing the transcription bubble, upstream and downstream footprints, the DNSA-RNA hybrid, and the RNA footprint. [Note, this info was at the top of a figure entitled “Adding nucleic acids to the structure”.] 4. What is the consensus promoter sequence for E σ 70 . 5. What are the kinetic intermediates in transcription initiation, and their footprints with DNAseI and KMnO 4 ? 6. Describe the experimental strategy for tracing the interactions of nucleic acids with polymerase in transcription complexes. 7.
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This note was uploaded on 02/17/2012 for the course CHEM 212 taught by Professor Staff during the Fall '10 term at Rutgers.

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transcription_studyguide - Study guide for Prokaryotic...

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