Unformatted text preview: 12 matrix of 96 wells, each of which are about 1 cm high and 0.7 cm in diameter. Known antigens are then fixed to the surface, incubated with dilutions of the patient’s serum, washed, and then reincubated with antibody to human IgG labeled with an enzyme. The enzyme activity is measured by adding substrate for enzyme and estimating the color reaction in a spectrometere. Illustration here: http://www.mabtech.com/main/Page.asp? PageId=26&PageName=About+ELISAm...
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This note was uploaded on 02/18/2012 for the course PAS 600 - 601 taught by Professor Garrubba during the Fall '10 term at Chatham University.
- Fall '10