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ELISA - 12 matrix of 96 wells each of which are about 1 cm...

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After several hours of searching, I was unable to find a clear description for this. I’ll keep looking but if you find one please let me know. Enzyme-linked immunosorbent Assay (ELISA, EIA) can be used for the quantitation of either antigens or antibodies in patient specimens. It’s based on covalently linking enzymes to a known antigen or antibody, reacting the enzyme-linked material with the patient’s specimen, and then assaying for enzyme activity by adding the substrate of the enzyme. So in other words .... The test is performed in a 8 cm x 12 cm plastic plate which contains an 8 x
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Unformatted text preview: 12 matrix of 96 wells, each of which are about 1 cm high and 0.7 cm in diameter. Known antigens are then fixed to the surface, incubated with dilutions of the patient’s serum, washed, and then reincubated with antibody to human IgG labeled with an enzyme. The enzyme activity is measured by adding substrate for enzyme and estimating the color reaction in a spectrometere. Illustration here: http://www.mabtech.com/main/Page.asp? PageId=26&PageName=About+ELISAm...
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