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Unformatted text preview: 12 matrix of 96 wells, each of which are about 1 cm high and 0.7 cm in diameter. Known antigens are then fixed to the surface, incubated with dilutions of the patients serum, washed, and then reincubated with antibody to human IgG labeled with an enzyme. The enzyme activity is measured by adding substrate for enzyme and estimating the color reaction in a spectrometere. Illustration here: http://www.mabtech.com/main/Page.asp? PageId=26&PageName=About+ELISAm...
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- Fall '10