Discussion 4 Fall 2010 - GENETICS (BIO 325) FALL 2010...

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GENETICS (BIO 325) FALL 2010 Discussion sheet 4 _____________________________________________________________________ ___ 1. A gal - mutant (a) Cannot grow without galactose (b) Is resistant to galactose (c) Can utilize galactose as a carbon source (d) Cannot utilize galactose as a carbon source (e) Can make its own galactose 2. A Str R mutant (a) Requires streptomycin (b) Can grow in the presence of streptomycin (c) Cannot grow in the presence of streptomycin (d) Makes its own streptomycin (e) Cannot make its own streptomycin 3. If a met - thr - Hfr strain is mated with an F - of genotype leu - thi - , prototrophic recombinants can be detected by plating the mixture on (a) Leucine and methionine (b) Threonine and thiamine (c) Leucine and thiamine (d) Methionine, threonine, leucine, and thiamine (e) Minimal medium 4. In an interrupted mating experiment in E. coli , three markers were selected for in exconjugants at the following time intervals: met + - 10 minutes; leu + - 12 minutes; arg + - 25 minutes. (a) Construct a linkage map showing the arrangement of these markers with respect to the origin of transfer and showing the direction of transfer. (b)
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This note was uploaded on 02/16/2012 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.

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Discussion 4 Fall 2010 - GENETICS (BIO 325) FALL 2010...

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