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Discussion 4 key Fall 2010

Discussion 4 key Fall 2010 - resulting in F’pro This F’...

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GENETICS (BIO 325) FALL 2010 Discussion sheet 4 - Key _________________________________________________________________________________ ____ 1. (d) 2. (b) 3. (e) 4. (a) arg + leu + met + origin 25 12 10 minutes (b) minutes (c) Hfr 5. (a) A (b) B (c) F + 6. arg + ade + thi + pro + glu + origin 36 27 17 11 6 minutes 7. (c) 8. 9. In the cross, Hfr (pro + ) x F - (pro - ), the F plasmid is excised imprecisely from the Hfr chromosome in the first step. The excised F carries pro + gene of the donor chromosome,
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Unformatted text preview: resulting in F’pro + . This F’ carrying the pro + gene is next transferred to the F-(pro-) cells early in the mating process. Cells carrying an F’pro + , when used in further matings, convert a majority of the F-(pro-) cells to F’pro + cells. 10. The gene order is trp-ala-pro . 1 try arg thy mal thi thr azi bio 2 met 4 his phe 1 3...
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