BLD430 Exam questions

BLD430 Exam questions - 1. It is normal to add loading dye...

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1. It is normal to add loading dye to a sample prior to loading it on a gel, it is purchased as a 6X stock. If the sample is 60 uLs and you want to load it all, how much loading dye would you add? A. 6 uL B. 10 uLs C. 12 uLs D. 15 uLs C. 12 uLs 60/5 = 12 2. A limitation of capillary electrophoresis is that you can only use low currents for electrophoresis because the large volume of matrix used. A. True B. False B. False 3. Pulse field electrophoresis is often used to separate very large neucleic acid pieces A. True B. False A. True 4. Once a gel has been prepared, loaded, and electrophoresis is complete, it will probably require some type of documentation, this can be accomplished by staining with: A. Brom cresol blue B. xylene cyanol C. Ethidium bromide D. eosin C. Ethidium bromide 5. If you wanted to prepare a 2.0% agarose gel that had a bed volume of 200 mLs, you would need: A. 0.4 grams of agar and 20 mLs of buffer
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B. 4.0 grams of agar and 200 mLs of buffer C. 0.4 grams of agar and 20 mLs of water D. 4.0 grams of agar and 200 mLs of water B. 4.0 grams of agar and 200 mLs of buffer 2gms / 100ul 6. If you needed to make a 6% acrylamide gel with a bed volume of 5 mLs and had a 30% acrylamide stock and 50X TAE, you would add: A. 1 mL aof acrylamide B. 0.1 mLs of acrylamide A. 1 mL aof acrylamide 6*5 = 30* ? 30/30=1 7. If you needed to make a 6% acrylamide gel with a bed volume of 5 mLs and had
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BLD430 Exam questions - 1. It is normal to add loading dye...

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