Restriction Enzymes

Restriction Enzymes - Brianna Haynes AG 3301 Dr Rahe...

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Brianna Haynes AG 3301 Dr. Rahe Restriction Enzymes: Found in bacteria, restriction enzymes’ are cutting enzymes that are gathered from bacteria and put into other organisms so we can see the effects the new DNA has on the organism; most often in antibiotic resistance. Normally, the bacteria use these enzymes as a defense mechanism for viral infections by disrupting the DNA of the invading virus. Out of the 3500 different types of restriction enzymes found, 150 are utilized commonly by researchers. Pancreatic cells are a type of eukaryotic organism that produces a similar enzyme that cuts but what makes restriction enzymes so efficient is that they read for a certain sequence of nucleotides. This certain group of nucleotides (usually 4-6 nucleotides long) that codes for the restriction enzyme to cut is called a recognition site, and the restriction endonucleases cut the recognition sites on both strands. Restriction enzymes are very useful in cloning also because they contain this ability to accurately cut DNA into fragments called recognition fragments. The function of the restriction endonucleases is to precisely cut and create a small set of
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This note was uploaded on 02/21/2012 for the course BIO 2450 taught by Professor Martin during the Spring '10 term at Texas State.

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Restriction Enzymes - Brianna Haynes AG 3301 Dr Rahe...

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