Lauren Taranowlmt8278 am DiscussionQuestions and Problems for Chapters 18 and 19 MaterialFrom Brooker - noneAdditional QuestionA2. Explain how to do each of the following procedures, what it accomplishes (why you would do it), and how it works.a.automated sequencingAutomated sequencing, similar to replication, is a way to determine the sequenceof a cloned gene, and requires a DNA polymerase and a primer. DNA is denatured by adding heat, and DNA polymerase adds a high concentration of dNTPs, as well as incorporating a low concentration of fluorescently-marked ddNTPs that terminate the nucleotide chain. The chain-terminating ddNTPs are randomly encorporated at every position along the insert. Frgments are separated in a PAGE gel, and as fragments run off the end of the gel, they are hit by a laser to excite the fluorophores attached to the ddNTP. The fluorescence is read by a detector, which reports the colors in the order they are emitted to tell us the unknown sequence of nucleotides.
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gene of interest, Blue white screening, DNA fingerprinting DNA, Lauren Taranow