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Steps>>Replication

Steps>>Replication - Steps of Replication...

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Steps of Replication Prokaryotes – circular, theta structure, 1 origin, bidirectional, has replisome 1 . DNaA box [DNaA protein] & AT-Rich regions – creates origin 2 . Helicase – enlarging replication bubble [forks] 3 . Topoisomerase – relieves tension, breaking phosphodiester bonds, unwinding strands, reconnects bonds 4 . Primase – 5’ to 3’ RNA polymerase activity, start of leading strand 5 . DNA polymerase III – 5’ to 3’ DNA polymerase activity, proofreading [3’ to 5’ exonuclease activity], high processivity on leading strand 6 . Primase – 5’ to 3’ RNA polyermase activity, start of lagging strands 7 . DNA polymerase III – 5’ to 3’ 5’ to 3’ DNA polymerase activity, proofreading [3’ to 5’ exonuclease activity], high processivity on lagging, Okazaki fragments 8. DNA polymerase I – 1) 5’ to 3’ exonuclease activity [removes primers on Okazaki fragments], 2) 5’ to 3’ DNA polymerase activity [fills in empty space with DNA], 3) 3’ to 5’ exonuclease activity [proofreading],
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