Exam 1 2011 - Name Student ID Zoo 470 Exam 1 Spring 2011...

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Name: _____________________________ Student ID:_________________ Page 1 Zoo 470 - Exam 1 - Spring 2011 – February 25, 2011 This exam has a total of 6 pages and a total of 50 points . You will have 90 minutes to complete it. Please put your name and student ID on all pages. Good luck! 1. Provide brief definitions for each of the following ( 5 points ): a. spermatid: b. Intracytoplasmic sperm injection (ICSI): c. acrosomal vesicle: d. transdifferentiation: e. fate mapping: 2. MPF and cytostatic factor are known to regulate meiotic cell divisions. Answer the following questions, based on your knowledge of these factors' effects on cell division. a. If purified CSF were injected into one cell of a two-cell embryo immediately after first cleavage, what would you predict would happen at the time of the next division? Clearly state your reasoning. ( 2 points ) b. Recall that frog eggs can be artificially activated by pricking them with a needle, initiating the same physiological changes as fertilization. What would you expect would happen to levels of MPF within a few minutes after pricking an egg? Clearly state your reasoning . ( 2 points )
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Name: _____________________________ Student ID:_________________ Page 2 3. You have isolated a gene that you believe encodes a novel sperm cell surface protein, headline , and you are making a presentation to a major pharmaceutical corporation for research support to work on this protein. a. You come to the presentation armed with the following pieces of data. For each of the following facts, state the technique(s) you used to obtain your data. If more than one technique is required, list all techniques necessary. ( 3 points ) Discovery Technique(s) Used for Discovery When viewed under the microscope, human sperm extracted from the epidydimis lack headline protein Excess headline protein in male zebrafish results in sperm that cannot fertilize eggs Although you do not possess a C. elegans headline mutant, you show that headline is required for fertilization in C. elegans b. headline mutant sperm are inefficient at undergoing the acrosome reaction. Suppose you find that headline encodes a calcium channel protein that allows calcium to enter sperm cells when they contact the zona pellucida. Describe one experiment that you could perform that would show that if the normal ionic events that trigger the acrosome reaction occur, the mutant sperm will undergo a normal acrosome reaction. ( 2 points ) c. Headline has multiple roles in development. You learn that
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Exam 1 2011 - Name Student ID Zoo 470 Exam 1 Spring 2011...

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