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Unformatted text preview: CHM 2201 Fall 2009 Department of Chemistry Organic Chemist ry Lab I Villanova University Page 1 of 4 Gas Chromatography Experiment 6A: Examination of an Unknown Mixture Week 3 September 8 14, 2009 From Bell , Clark and Taber, pages 64, 65 1. Choose one of the unknown liquid alcohol mixtures ( Unknown 1 or 2 ) for GC analysis and place 5 drops in a small labeled vial. a. Each unknown mixture will be composed of two of the following alcohols: i. Methanol ii. 1-Propanol iii. 1-Butanol iv. 1-Pentanol 2. Obtain and record in your notebook the retention times (R t ) which have been determined for the pure alcohols (shown above) by the TAs specifically for your class. You can obtain the R t values for the known alcohols from the gas chromatogram charts displayed in the instrument room. 3. Also record in your notebook the GC oven temperature, gas flow rate and column used for obtaining the above R t s. Be sure that the R t s you obtain are for the GC machine that you actually use for your analysis. 4. Have a TA inject your unknown sample into the GC. 5. Use the gas chromatography chart of your unknown to deduce which liquids are in your unknown by comparison of R t s of the known compounds with those of the unknown mixture. 6. Place a copy of your chromatogram in your notebook. Be sure to record oven temperature, gas flow rate and column used. Thin Layer Chromatography Experiment 8B: TLC of Analgesic Drugs; Determination of an Unknown Bell, Clark and Taber, pages 76 - 78 1. In the community hood, there are solutions of 4 reference compounds that may be ingredients in the unknown analgesic preparations. The reference compounds are: a. Aspirin (acetylsalicylic acid) b. Salicylic acid c. Acetaminophen (Tylenol ) d. Caffeine 2. Place 2-3 drops (you dont need much material for your analysis!!) of each of the above reference compounds into a small labeled test tubes or vials and take to your bench. CHM 2201...
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