Bio Lab Report

Bio Lab Report - Testing for nucleic acids Dische...

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Testing for nucleic acids: Dische Diphenylamine Test Hypothesis: Among the given samples, which are water, DNA, and RNA, DNA is the only one expected to test positive with diphenylamine, because DNA contains the sugar deoxyribose that reacts with the diphenylamine reagent when heated. Purpose: The purpose of this experiment is to find whether all the solutions containing nucleic acids will test positive with diphenylamine and if not why? Water in this case can be taken as a control for it contains no nucleic acids. Background: Nucleic Acids, RNA & DNA, are molecules composed of many nucleotides bonded together in long strands. Each nucleotide contains a five-carbon sugar as part of its structure. Specifically, DNA contains the contains the sugar deoxyribose that reacts with diphenylamine reagent when heated forming a blue color, while RNA contains uracil. Procedure: 1.) 3 test tubes marked at 2cm and 4cm were obtained and labeled 1, 2, 3. 2.) In each of the test tubes, a solution was poured to the 2cm mark as follows:
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This note was uploaded on 02/20/2012 for the course H/A 121 taught by Professor Alamo during the Spring '12 term at Morgan.

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Bio Lab Report - Testing for nucleic acids Dische...

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