5491 - Pleural fluid cell block from nonfatal case Abundant...

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Pleural fluid cell block from nonfatal case Abundant Bacillus anthracis granular antigen staining inside mononuclear inflammatory cells. Immunohistochemical assay with mouse monoclonal (Mab) anti- B . anthracis capsule antibody. Detection with alkaline phosphatase and naphthol fast red. Original Magnification 158X
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Mediastinal lymph node from a fatal case Extensive capsular and sinusoidal hemorrhage. Hematoxilin-Eosin stain. Original Magnification 25X
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Lymph node from same fatal case as the previous slide Abundant B. anthracis granular antigen inside mononuclear inflammatory cells . Some bacilli (arrows) in the subcapsular hemorrhagic area. Immunohistochemical assay with mouse monoclonal (Mab) anti- B . anthracis cell wall antibody. Detection with alkaline phosphatase and naphthol fast red. Original Magnification 100X
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Lung tissue from a fatal case B. anthracis granular antigen staining inside a perihilar macrophage (red arrow). Intra- and extracellular bacilli (black arrow). Immunohistochemical assay with mouse monoclonal (Mab) anti- B . anthracis cell wall antibody. Detection with alkaline phosphatase and naphthol fast red. Original Magnification 100X
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McFadyean's reaction showing short chains of Bacillus anthracis cells lying among amorphous, disintegrated c apsular material. White blood cells can also be seen. A closer look at the pathogenesis of Anthrax
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pXO2 pXO1 Virulence Factors of B. anthracis pXO1 plasmid (110 MDa) : codes for Anthrax toxin (in 3 parts) : PA : Protective antigen : 82.7 kDa - Forms Heptamer EF : Edema Factor : 88.9 kDa - Adenyl Cyclase LF : Lethal Factor : 90.2 kDa – Metalloprotease pXO2 plasmid (60 MDa) : codes for poly-D-glutamate capsule
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Virulent Strains: both plasmids Cap+ Tox+ Attenuated Strains: no pXO2 Cap- Tox+ Attenuated Strains: no pXO1 Cap+ Tox- Anthrax Strains Pasteur (grown at 43 ° C) Cap+ Tox- Some Cap+ Tox+ Sterne (cattle vaccine) Cap- Tox+ Whole viable Human – Acellular Al(OH) 3 - adsorbed Cap+ Tox+ Grown to yield
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This note was uploaded on 02/22/2012 for the course HIST 312 taught by Professor Staff during the Fall '10 term at Rutgers.

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5491 - Pleural fluid cell block from nonfatal case Abundant...

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