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Lecture 5 - animal makes antibodies and the antibodies bind...

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Lecture 5 January 13, 2012 64-77; 92-100 Learning Outcomes 1. Compare the structures and physical properties of PrP C and PrP Sc . Describe the model for “infection” PrP C - digested by enzymes, alpha helices PrP Sc - can influence PrP C PrP Sc , Beta sheets (can’t clump together), forms large aggregates Primary structure the same for both, but secondary structure changes when it comes to PrP Sc 2. Define proteomics. List three experimental approaches used in proteomics and explain what they do. Proteomics- studying proteins to determine function 2-D gel electrophoresis- it has a pit gradient and proteins separate depending on PI(isoelectric point). SDS page gel separates proteins by molecular weight- smaller proteins go lower on gel coimmunoprecipitation- takes foreign proteins and puts it in animals and
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Unformatted text preview: animal makes antibodies and the antibodies bind to certain proteins; it helps see what protein-protein interactions occur-cleave with proteases and see what proteins bind protein micro array- can tell which interactions occur w/ proteins 3. Explain why biological catalysts are needed in a cell (as opposed to other means to speed up reactions). They don’t effect energy required to carry out equilibrium, lowers activation energy, induce strain so easier to break, makes sure the right reaction occurs. 4. Explain why a reaction which results in products with lower energy than reactants will not occur spontaneously. Because energy is absorbed- the reaction in low energy don’t have enough energy to over come activation energy....
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