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Unformatted text preview: . Explain the value of the L-B plot over the M-M plot. Vmax and Km are difficult to evaluate from a Michaelis-Menten curve take reciprocal to linearize. 4. Illustrate the M-M and L-B curves that would result from reducing the enzyme concentration. 5. Explain the difference between types of reversible inhibitors and illustrate each on M-M and L-B plots. Competitive inhibitors- reversible inhibitors that compete with a substrate for access to the active site for an enzyme Noncompetitive inhibitors- the substrate and inhibitor do not compete for the same binding site; generally, the inhibitor acts at a site other than the enzyme’s active site. Illustrated in Notebook....
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- Spring '12
- Enzyme, Enzyme inhibitor, substrate concentration, enzyme kinetics experiment, theoretical saturation point