01 Lab UNIT 1 Wi11 - Lab #1: Aseptic Technique and Streak...

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Lab #1: Aseptic Technique and Streak Plate Method Read: Ex 1-3 Common Aseptic Transfers and Inoculation Methods and Ex 1-4 Streak Plate Method (p. 17-27) Learning Objectives: The student will be able to: 1) Understand and apply principles of aseptic technique 2) Implement an appropriate procedure for decontamination & clean-up of lab spills. 3) Identify an inoculating loop and a Bacti-Cinerator and describe how to use these appropriately 4) Appropriately label a bacterial culture 5) Describe how to transfer bacterial organisms aseptically from one culture to another, including broth, slant, and plate cultures 6) Explain the purpose, principle, and procedure for the streak plate technique (quadrant method) 7) Explain why plates are incubated in an inverted position 8) Distinguish pure and mixed cultures 9) List considerations for safe handling of bacterial cultures In Lab: the bacterial organisms used this lab are Escherichia coli and Staphylococcus aureus 1) Aseptically transfer a bacterial culture from a broth to a broth and from a broth to a slant 2) Aseptically transfer a bacterial culture from a plate to a broth and from a plate to a slant 3) Streak a plate with a bacterial culture using the quadrant method Lab Notebook: 1) What is the purpose of aseptic technique? 2) What 3 pieces of information should be included in the label for every culture? Where should a broth, a slant, and a plate be appropriately labeled and why? 3) Very briefly, note all the steps in making a broth to broth transfer, using aseptic technique. 4) Draw a circle about 3 inches in diameter, representing an agar plate. Diagram the pattern you would use to streak the plate using the quadrant method. Put a star at points where you would flame your loop. 5) What is the purpose of the streak plate technique for inoculating an agar plate? 6) What might be the problem if you incubated your plate in the upright position? 7) After incubation, did all your broths and slants grow? How might you explain it if your culture did not grow? 8) Can you tell, or how can you tell if a culture is pure when grown in a broth? on a slant? on a plate streaked using the quadrant method? 9) Sketch your streak plate after incubation. Was your technique successful? Explain why or why not. Critique your streak technique, including what you would do next time to improve your technique.
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Lab #2: Microscopy and Microorganisms in the Environment Read: Ex 3-1 Introduction to the Light Microscope (p. 70-75) and Ex 2-1 Ubiquity of Microorganisms (p. 34-35) Learning Objectives: The student will be able to: 1) Give the name, function, and location of the parts of our compound light microscopes: Arm Base Power cord Stage Condenser lens Light switch Iris diaphragm Ocular lenses Nosepiece Objective lenses (4) Voltage control knob Coarse focus knob Fine focus knob Stage adjustment knobs 2) Define and use the following terms related to microscopy: Light microscope Brightfield microscopy Compound microscope Magnification
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This note was uploaded on 02/21/2012 for the course MICROBIOLO BI127 taught by Professor Leunk during the Spring '09 term at Grand Rapids Community College.

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01 Lab UNIT 1 Wi11 - Lab #1: Aseptic Technique and Streak...

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