Lab 8 - phototoxicity agai2003 mode

Lab 8 - phototoxicity agai2003 mode - Phototoxicity of...

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Phototoxicity of secondary metabolites Ioana Dobre
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Phototoxins = light activated toxins (molecules that upon absorption of a photon, their structure get altered) They have no known function in primary metabolic processes like photosynthesis, nitrogen assimilation etc. Instead are show to function as plant defence against fungi, bacteria, higher animals How could they have evolved? Coevolution between plants and herbivores or other pests Selection pressure from these pests favored the production of new, increasingly complex and toxic photochemicals Experiment: Experiment: - We looked at 8-methoxypsoralen, one group of phototoxins - We compared its antifungal activity to that of actual plant tissue samples We determined: - Which plants contained phototoxins - Which plant organs contained phototoxic compounds - Relative degree of toxicity in plant tissue sample and in a pure 8- methoxipsoralen extract by using a yeast lawn assay - Whether or not plants showed phototoxicity in the absence of light
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This note was uploaded on 02/21/2012 for the course BIO Bio2137 taught by Professor Namroud during the Fall '11 term at University of Ottawa.

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Lab 8 - phototoxicity agai2003 mode - Phototoxicity of...

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