Lab_8[1] - Lab 8: Isolation and Phototoxicity of Secondary...

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Lab 8: Isolation and Phototoxicity of Secondary Metabolites By Yousef Al-Enzi 5259294 Partner: Ainura Isina BIO2137 Section A2 Demonstrators: Elizabeth Da Silva Rebbeca Assaf November 25 th , 2009 Department of Biology University of Ottawa
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Table 8.1: The effect of light on the relative degree of phototoxicity in 4 extracts: 95% ethanol, hexane, ethanol-H 2 O, and root residue using a yeast lawn bioassay ( Saccharomyces cerevisiae ) following 48 hours of growth. The average diameter of the inhibition zones (mm) is measured and individual data with the means (± SE) of 53 counts are presented. a- Exposed to UV light for 1 hour followed by 48 hours of incubation in the dark b- Incubated in the dark immediately for 48 hours Class Inhibition Zone (mm) [Standard error] Individual Inhibition Zone (mm) Experimental a Control b Experimental Control 95 % Ethanol 16.84 [2.9] 2.48 [0.6] 11 0 Hexane 14.65 [1.7] 0.66 [0.5] 14 0 H 2 O Ethanol 8.28 [2.7] 0.51 [0.3] 0 0 Residue Tagetes erecta L. 18.25 [3.1] 0.89 [0.5] 10 0
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plants: Bidens sp. (n=15), Citrus sp. (n=6), Tagetes erecta (n=7) and Vinca rosea (n=9); and 8- methoxypsoralen (n=13), using a yeast lawn bioassay ( Saccharomyces cerevisiae ) following 48 hours of growth. The means (±SE) of the diameter of the inhibition zone in (mm) is measured are shown along with individual data for Tagetes erecta are shown. Experimental Inhibition Zone (mm) [standard error] Control Inhibition Zone (mm) [standard error] Flower Leaf Stem Root Flower Leaf Stem Root Individual’s Tagetes erecta (marigold) 0 0 0 17 0 0 0 0 Bidens sp. (golden eye) Citrus sp. (lemon tree) 7.27 [2.3] 0.0 [0.0] 0.27[0.3] 0.0 [0.0] 3.40[1.7] 0.0 [0.0] 4.77 [1.5] 0.0 [0.0] 0.14 [0.1] 0.0 [0.0] 0.0 [0.0] 0.0 [0.0] 0.0 [0.0] 0.0 [0.0] 0.07[0.1] 0.00 [0.0] Tagetes erecta (marigold) 6.6 [5.8] 1.7[1.1] 2.40[1.7] 12.0 [4.2] 0.14 [0.0] 0.0 [0.0] 0.4 [0.4] 0.07[0.0] Vinca rosea (periwinkle) 0.0 [0.0] 0.56 [0.6] 0.0 [0.0] 2.94 [2.1] 0.0 [0.0] 0.0 [0.0] 0.0 [0.0] 0.00 [0.0] 8- methoxypsoralen 14.0 [0.4] 0.0 [0.0] a- Exposed to UV light and visible light for 4 hours followed by 48 hours of incubation in the dark. b-
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Lab_8[1] - Lab 8: Isolation and Phototoxicity of Secondary...

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