bio344FinalMaterial - Exam 1 1. Entrez/Pubmed AND How to...

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Exam 1 1. Entrez/Pubmed AND How to design an experiment. What are controls? -Plasmid: A circular DNA that replicates independently of the cell’s chromosomes. - Restriction enzyme/endonuclease: An enzyme that recognizes specific base sequences in DNA and cuts at or near those sites. 2. Summary of bacterial transcription (Ch. 6) Denaturation: Breaking of H-bonds of a whole strand, separating the two strands. (melting: denaturation of part of the strand) Annealing: base-pairing between two nucleic acid strands. Enzymes go 5’ to 3’ (chemists go 3’ to 5’) THE bacterial RNA polymerase: core ( α , α , β , β ’)+sigma ( σ ) 3. 4. pg. 250-256, the 3 eukaryotic RNAp’s: I for rRNA, II for hnRNA’s and snRNA’s (found by treatment with α -amanitin; 12 subunits found through epitope tagging, immunoprecipitation, SDS-PAGE detergent and electrophoresed by autoradiography) 5. Eukaryotic transcriptional activators (Ch.12 part 1) Terms: 5’ to 3’, promoter, core promoter, closed complex, open complex(tighter). Promoter clearance, repressor, activator.
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This note was uploaded on 02/22/2012 for the course BIO 344 taught by Professor Herrin during the Spring '08 term at University of Texas at Austin.

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bio344FinalMaterial - Exam 1 1. Entrez/Pubmed AND How to...

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