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344Exam1 - denaturation The breaking of H-bonds that...

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denaturation The breaking of H-bonds that results in the separation of two strands of DNA. Separation of two strands of a DNA double helix or the severe disruption without breaking the covalent bonds of its chains. melting The breaking of H-bonds that results in the separation of two strands of DNA. The difference between melting and denaturation is that melting refers to small portions of DNA while denaturation refers to the whole strand . Enzymes typically melt DNA while boiling and harsh chemical treatments denatures it. annealing . Refers to base-pairing between two nucleic acid strands. reannealing . When a single stranded nucleic acid re-establishes base pairs that it previously had, prior to melting, it is said to reanneal. renaturation is the equivalent term used when the molecule was denatured. hybridization . When two nucleic acid molecules are allowed to anneal to one another. Usually used to refer to the instance when two non-identical molecules are allowed to pair up. Typically one is a probe and one is a naturally occurring molecule.
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