126LSYL F 08 -1 - SYLLABUS FOR BIO 126 L (Fall 2008) Dr....

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SYLLABUS FOR BIO 126 L (Fall 2008) Dr. Pratibha Saxena You need to be either co-registered in BIO 326R/BIO 226N or have taken their equivalent earlier Class website: http://www.sbs.utexas.edu/psaxena/ SCHEDULE OF LAB EXERCISES WEEK STARTING LAB Sep 1 Lab check-in Guidelines for success Cleanliness and safety Sep 8 Lab 1: Use of the light microscope (video-1) Sep 15 Ubiquity of microorganisms (requires coming to lab the next day) Appendix A: Sterilization Lab 2: Pure culture techniques (video-2, 2 clips) Turn in practice streak plate Sep 22 Lab 3: Selection and differentiation (requires coming to lab for the next 2 days) Graded Streak Plate (20 points) Sep 29 Lab 4: Dilution and plating (video-4, 3 clips) (requires coming to lab the next day) Colorimetry and turbidimetry (Graph 10 points) Oct 6 Lab 5: Identification of organisms (video-5, 5 clips) (requires coming to lab for 4 days) Mixed culture unknown (100 points) Oct 13 Labs 6: Bacterial growth I (Graph 20 points) (requires coming to lab the next day) Oct 20 Growth Curve Lab (Graphing) (takes 10 days but there is some flexibility) Lab 8: Enrichment culture & Review Oct 24 EXAM I Mon Labs Welch 2.224 (Fri) Tues Labs Welch 3.502 5:30p.m. - 7:00p.m. Covers Labs 1-5 Wed Labs Welch 1.308 Th Labs Welch 1.316 Oct 27 Lab 9: Phenotypic variation, isolation and (requires coming back on the 2nd and 4th day) characterization of bacterial mutants Nov 3 Lab 10: Pathogenic bacteria (requires coming to lab for 4 days) Unknown mixture (120 points) Nov 10 Lab 11: Phage and DNA (video-11) (requires coming to lab the next day) (Graph 10 points) Nov 17 Nov 21 EXAM II Mon Labs Welch 2.224 (Fri) Tues Labs Welch 3.502 5:30p.m. - 7:00p.m. Wed Labs Welch 1.308 Th Labs Welch 1.316 (Worth 20 points of technique/participation) Have your TA check and record that you cleaned out your incubator basket and hall locker
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SUPPLIES AND EQUIPMENT REQUIRED FOR EACH STUDENT 1. Laboratory manual: available at Speedway Printing in Dobie Mall 2. Lock for hall locker (to be purchased separately) 3. Inoculating wire holder with knurled chuck (wire will be supplied) 4. Pre cleaned microscope slides (2 doz.) 5. Cover slips No. 1 (1 doz.) 6.
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This note was uploaded on 02/22/2012 for the course BIO 126L taught by Professor Saxena during the Fall '08 term at University of Texas at Austin.

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126LSYL F 08 -1 - SYLLABUS FOR BIO 126 L (Fall 2008) Dr....

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