Lab 5 - Heather Oslund 10-6-08 Mon 2-5 BME 2.106...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Heather Oslund 10-6-08 Mon 2-5 BME 2.106 Desk/Locker/Incubator #16 Lab 5: Identification of organisms Purpose To learn the processes of biochemical testing to determine the identity of an unknown organism. Discussion Each of the eight biochemical tests helped determine a different characteristic of the organism. In narrowing down the possibilities for my unknown organism I first used the gram reaction and cell morphology (negative and short rods). Through these observations I could exclude half of the candidate organisms, leaving Alkaligenes faecalis , Pseudomonas flourescens , Escherichia coli , Klebsiella pneumoniae , and Proteus vulgaris . I then observed the four carbohydrate fermentation tests. The glucose test was yellow in color and there was a large bubble in the durham tube, indicating that this organism fermented the glucose, producing acid (lower pH) and gas (indicative of an aerogenic organism) products. These results allowed me to narrow the possibilities down to only two organisms,
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 02/22/2012 for the course BIO 126L taught by Professor Saxena during the Fall '08 term at University of Texas at Austin.

Ask a homework question - tutors are online