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127 - 330 Class Notes Manipulating and Analyzing Nucleic...

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330 Class Notes Manipulating and Analyzing Nucleic Acids 1.27.12 Slide 2: DNA Modifying Enzymes Ligase involved in joining nucleotides together. Slide 3: Nucleases-Exonucleases Recognize ends of DNA and will move consecutively down the DNA molecule, cleaving the phosphodiester bonds between sugar residues. DNase I is not very specific. DNase have specific Slide 4: Restriction Endonucleases Recognize specific sequences within DNA molecules. Pallindromes…Read the same forward and backwards Overhangs…create sicky ends, helpful to join two pieces of DNA. Slide 5: Table 1.3 Slide 6: Restriction Enzyme Mapping Can use multiple restriction enzymes and based on how many fragments/ cuts created with each enzyme, you can map out the genome yourself. Not used too much anymore, due to DNA sequencing programs. Slide 7: RFLPs or RFPs Good to see differences observed in different populations of same bacteria. One treated without mutagen and the other treated with mutagen and observe differences.
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