30661 - Sanjeev Sharma*, Aarti Bhardwaj $ , Shalini Jain #...

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Unformatted text preview: Sanjeev Sharma*, Aarti Bhardwaj $ , Shalini Jain # and Hariom Yadav # *Animal Genetics and Breeding Division, # Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, India INTRODUCTION INTRODUCTION Preselection of sex Earlier approaches superstition based Modern techniques make it possible now Either X-Y sperm sorting or Embryo sexing RELEVANCE RELEVANCE Large hue and cry against prenatal sex determination In animal science the technology can be a great boon Progressive & rich dairy farmers are now willing to spend money for new technologies to improve profitability of their herd METHODS OF SPERM SEPERATION Based on physical differences HY antigen Albumin gradient & percoll gradient Electrophoretic separation (motility loss) Flow cytometry (most successful) DRAWBACKS OF FLOW CYTOMETRY DRAWBACKS OF FLOW CYTOMETRY Very costly Decreased viability of spermatozoa Less number of spermatozoa sorted per hour (3.5x 10 5 ) Jafar & Flint, 1996 Will not be economically viable till 2005 Amman, 1999 SEX DETERMINATION IN EMBRYO Sex determined in pre-implantation embryos Approach- either invasive or non invasive Splitting of sexed embryos First successful embryo sexing done by Gardner, 1968 in rabbits by cytological method (Barr body observation) VARIOUS METHODS OF EMBRYO SEXING Non invasive Methods-- Immunological assay of HY antigen Quantification of X-linked enzyme William et al., 1986 Differential growth of male & female embryo Yadav et al., 1992 Invasive Methods-- Cytogenetic analysis--observing Barr bodies--chromosome analysis Y-specific DNA probe Y-specific DNA primer & PCR BARR BODY OBSERVATION BARR BODY OBSERVATION Barr body forms after certain stage in embryo...
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30661 - Sanjeev Sharma*, Aarti Bhardwaj $ , Shalini Jain #...

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