38251 - n ) fibroblasts myofibroblasts EGF ( E ) TGF-β ( G...

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AVNER FRIEDMAN Mathematical Biosciences Institute Ohio State University, Columbus, USA
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● Epithelial cells are transformed by gene mutations ( TEC s). ● TECs secrete TGF-ß (transforming growth factor beta). ● TGF-ß signals to fibroblasts which get transformed to myofibroblasts; both secrete EGF (epidermal growth factor). Fibroblasts/myofibroblasts form the microenvironment of the tumor We want to examine how they interact with the TECs at the very early stage of cancer. Thin slice of a breast tumor http://www.jamesline.com/images/frontiers/summer2005/PartnersInCrime1.jpg Background on Breast Cancer
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EGF , RAS, MAPK TGF- β , SMAD Complex pathways stimulating cell proliferation biocarta.com
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TRANSFORMED EPITHELIAL CELLS (TECs)
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X = 0 MEMBRANE EGF ( E ) TGF- β ( G ) fibroblasts ( f ) myofibroblasts ( m ) TEC (
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Unformatted text preview: n ) fibroblasts myofibroblasts EGF ( E ) TGF-β ( G ) EXPERIMENT & MODEL DOMAIN ● Place TECs in solution at bottom of well. ● Place semi-permeable membrane above the solution. ● Deposit fibroblasts/myofibroblasts above the membrane. ● Count the number of TECs in day 2, 4, 6, and 8. ● TGF-ß and EGF can cross the membrane, but cells cannot. ( TECs ) MEMBRANE Ω-Ω + Diffusion of TECs CHEMOTAXIS : n cells move towards increasing concentration of EGF Cell proliferation : logistic growth enhanced by EGF via Hill-type kinetics production of EGF by fibroblasts & myofibroblasts in Ω-on the semi-permeable membrane ● Having gained understanding of the early stage of breast cancer in vitro without ECM, the next step is to include ECM, using the value of γ that was established....
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This note was uploaded on 02/23/2012 for the course SCI 310 taught by Professor Staff during the Fall '10 term at Rutgers.

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38251 - n ) fibroblasts myofibroblasts EGF ( E ) TGF-β ( G...

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