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Fundamentals of Biochemistry: Life at the Molecular Level

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Chemistry 3785 Sample Final Exam Name KEY 200 points Points Score Points Score I 84 VI 14 II 10 VII 16 III 18 VIII 12 IV 18 IX 12 V 16 Total 200 I. (84) Multiple Choice. Circle the letter corresponding to the best answer. Three points each 1. Fructose in muscle is converted to the glycolytic intermediate(s) a. fructose-6-P b. fructose-1,6-bisphosphate c. dihydroxyacetone phosphate and glyceraldehyde-3-P d. 2 molecules of dihydroxyacetone phosphate e. 2 molecules of 1,3-bisphosphoglycerate 2. Which transformation of gluconeogenesis shown below requires a different enzyme than that used in glycolysis? a. 1,3 -Bisphosphoglycerate Glyceraldehyde-3-phosphate b. Glucose-6-phosphate Glucose c. Fructose-1,6-bisphosphate Fructose-6-phosphate d. All the above e. b and c 3. Avidin, a protein found in egg white, has a very high affinity for the coenzyme, biotin. Which enzyme catalyzed transformation shown below would be inhibited in the presence of avidin? a. Pyruvate → Oxaloacetate b. Oxaloacetate → Phosphoenolpyruvate c. Lactate → Pyruvate d. Glucose → Lactate e. Both a and b 4. How many high energy phosphoanhydride bonds are consumed in the conversion of 2 pyruvate to 1 molecule of glucose via gluconeogenesis? a. 2 b. 3 c. 4 d. 6 e. 8 5. The bifunctional enzyme which synthesizes frucotse-2,6-bisphosphate is inhibited by a. fructose-6-P b. covalent modification c. ATP d. AMP e. None of the above 6. Which of the following statements about the enzyme complexes of the electron transport chain are correct? Page 1 of 11
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a. Three of the four complexes pump protons from the cytosolic to the matrix side of the inner mitochondrial membrane. b. They are integral membrane proteins located in the inner mitochondrial membrane. c. They interact with each other via mobile electron carriers. d. All the above are correct. e. only c and d are correct 7. Which of the following statements about the chemiosmotic theory is false ? a. Electron transfer in mitochondria is accompanied by an asymmetric release of protons on one side of the inner mitochondrial membrane. b. Energy is conserved as a proton gradient. c. The effect of uncoupling agents is a consequence of their ability to carry protons through membranes. d. Oxidative phosphorylation cannot occur in membrane-free preparations. e. The O conformation of the αβ protomer of ATP synthase is catalytically active. 8. The rate of flow of electrons through the electron transport chain is most directly regulated by a. the ATP:ADP ratio. b. the concentration of acetyl CoA. c. the rate of oxidative phosphorylation. d. feedback inhibition by H 2 O. e. the catalytic rate of cytochrome oxidase. 9. Uncoupling of oxidative phosphorylation a. halts all oxidative metabolism. b.
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PracticeExam - Chemistry 3785 Points I II III IV V 84 10 18...

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