10-28 - We'd dub the one that came off best into the final...

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We'd dub the one that came off best into the final transcription . It gave us a chance to ad lib as much as we wanted, knowing that excess ad libbing could be s(p)liced from the final product. Bing Crosby , rogue geneticist?
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Announcements New quiz available today. Due Wednesday, November 2 at noon.
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Types of damage experienced by DNA Ionizing radiation can break DNA backbone Exposure to a variety of reactive chemicals, some made by cell metabolism, can alter DNA bases structurally Ethyl methansulfonate (EMS) is a chemical mutagen that creates point mutations and is used as a research mutagen Ultraviolet radiation causes adjacent pyrimidines (C,T) to interact covalently, forming a dimer Absorption of thermal energy generated by metabolism in a warm- blooded bird or mammal can split adenine & guanine from their attachment to DNA backbone sugars Pyrimidine dimer
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Three Steps That Give Rise to High-Fidelity DNA Synthesis REPLICATION STEP ERRORS PER NUCLEOTIDE POLYMERIZED 5′→3′ polymerization (standard replication) 1 × 10 5 3′→5′ exonucleolytic proofreading (DNA polymerase I) 1 × 10 2 Strand-directed mismatch repair 1 × 10 2 Total 1 × 10 9 Alberts et al., Molecular Biology of the Cell
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Correct pairing (A-T or G-C) must occur between template and base in order to be incorporated into new strand in polymerase active site In most cases, incorrect pairings will not be accepted, but sometimes (1/100,000 bp), they are, causing a mismatch error DNA Polymerase I : Online 3’ to 5’ DNA repair functions
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Nucleotide excision repair in prokaryotes 4 proteins UVRA,UVRB, UVRC, and UVRD contribute. UVRA and UVRB scan DNA and sense damage UVRC cuts out a section of DNA that includes the damaged part UVRD is a helicase that separates the DNA, releasing the damaged DNA for degradation. DNA polymerase I and DNA ligase fill in the proper sequence and seal the DNA strand. Cooper GM . The Cell: A Molecular Approach. 2nd edition.
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Base excision repair (BER) If a base has been incorrectly placed, it can be removed by DNA glycosylases. Glycosylases cleaves glycosidic bond and remove base only, not nucleotide or nucleotide sequence as in NER. The backbone is preserved.
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This note was uploaded on 02/23/2012 for the course BIO 230 taught by Professor .. during the Spring '10 term at Purdue University.

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10-28 - We'd dub the one that came off best into the final...

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