{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

13941 - One Strategy for Gene/Cell Therapy Harness the...

Info icon This preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
One Strategy for Gene/Cell Therapy: Harness the Natural Ability of Adult Stem Cells to Repair Tissues Darwin J. Prockop, M.D., Ph.D., Director, Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
How does the body repair itself? Cohnheim (1867): ---probably by cells that come from the blood stream. More recent hypothesis ---probably first by the stem-like cells found in many tissues. ---probably after these are exhausted ---by stem cells from the bone arrow
Image of page 2
How well does the repair system work? We are not at the top of the tree of evolution: Hydra, planaria, etc. ---complete organism from a few cells. Salamanders and related ---complete limbs or tails Mice and rats ---difficult to produce permanent damage in most organs.
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
One Strategy for Gene/Cell Therapy Use stem-like cells from bone marrow ---to make man more like a rodent or perhaps salamander. Our approach ---large numbers of a patient’s own cells.
Image of page 4
Bone marrow aspirate ---Local anesthesia Skeletal and Related Disease Diseases of CNS Marrow stromal cells ---expanded ---gene engineered Therapy of Same Patient
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Questions 1. Are we certain that marrow contains stem cells for non-hematopoietic tissues? 2. If so, which cells? 3. If so, how do the cells repair tissues?
Image of page 6
Questions 1. Are we certain marrow contains stem cells for non-hematopoietic tissues ? Conclusive date now from a. differentiation of cells in culture. b. trials in animals. b. patients with bone marrow and organ transplants. (see Prockop et al. PNAS 100(1): 11917- 23, 2003)
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
2. Which Cells from Marrow? 1. Hematopoietic stem cells (CD 34* or CD 133*)? 2. “Side population” cells ? 3. Multipotential adult progenitor cells ? 4 . Mesenchymal stem/marrow stromal cells(MSCs)? OR some still unidentified cell?
Image of page 8
One Candidate for Reparative Cell from Bone Marrow Isolated by ---plating bone marrow on plastic. Most common names: ---“ marrow stromal cells ---used as feeder layers for HSCs. ---“ mesenchymal stem cells ---differentiate into most cell types.
Image of page 9

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern